Cell Growth Stimulation, Cell Cycle Alternation, and Anti-Apoptosis Effects of Bovine Bone Collagen Hydrolysates Derived Peptides on MC3T3-E1 Cells Ex Vivo

Cell Growth Stimulation, Cell Cycle Alternation, and Anti-Apoptosis Effects of Bovine Bone Collagen Hydrolysates Derived Peptides on MC3T3-E1 Cells Ex Vivo

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Bovine bone collagen hydrolysates promote bone formation through regulating bone growth.However, the peptide sequences within these isolates have not been characterized.In this study, twenty-nine peptides from bovine bone collagen hydrolysates were purified and identified using nano-HPLC-MS-MS and Peak Studio analysis.

HHGDQGAPGAVGPAGPRGPAGPSGPAGKDGR (Deamidation) and GPAGANGDRGEAGPAGPAGPAGPR (Deamidation) enhanced cell viability, inhibited apoptosis, and significantly altered the cell Countertop Deep Fryers cycle of MC3T3-E1 osteoblast cells.These peptides were selected to perform molecular docking analysis to examine the mechanism underlying these bioactivities.Molecular docking analysis showed that Grill / Griddle Screens these two peptides formed hydrophobic interactions and hydrogen bonds with epidermal growth factor receptor (EGFR) to activate the EGFR-signaling pathway, which may explain their bioactivity.

These findings indicate that these and other similar peptides might be candidates for the treatment of osteoporosis.